| Abstrak/Abstract |
Pumpkin seeds contain lignan phytoestrogens, which have the potential to retrievemenopausal syndromes caused by estrogen deficiency. This study aims to determinethe estrogenic effects of the extract of pumpkin seeds (EPS). In vitro experimentwas performed by 3?(4,5?dimethylthiazol?2?yl)?2,5?diphenyltetrazolium bromide (MTT)assay on CHO?K1 cells. In vivo experiments were conducted using 24 of 7?weeks?oldfemale Sprague Dawley rats, divided into four treatment groups (ovariectomized[OVX], ovariectomized for treatment with 2??M17??estradiol, ovariectomized for treat-ment with 500 mg/kg BW EPS, and ovariectomized for treatment with 1000 mg/kg bodyweight (BW) EPS) and two baseline groups (OVX and sham). Uterine weight, mammaryglands, serum lipid, and bone density were observed after 30 days. In silico experimentwas conducted through molecular docking of secoisolariciresinol and lariciresinol to theestrogen receptors (ERs). EPS exhibited a biphasic effe ct, inducing cell growth at10–100 ?g/mL and decreasing cell viability at 250–1000 ?g/mL. EPS 1000mg/kg BW improved uterine weigh t and retrieved mammary glands comp arable with17??estradiol. Meanwhile , EPS 500 and 1000 mg/kg BW increased high?dens ity lipo-protein (HDL), decreas ed low?densi ty lipoprotein (LDL), and recovered bone densityin correlation with the decreasing osteoclasts and increasing osteobla sts. The dockingscore of secoi solariciresinol with ER? and ER? were ? 91.13 and ?95.87, respectively,and lariciresinol were ?80 .91 and ? 90.29, respectively. These results demonstratedthat EPS performs estrogenic properties in rats model and potential to overcomesymptoms caused by estrogen deficiency. |
