Studies on Antihyperglycemia and Antihypercholesterolemia in Rats (Rattus norvegicus) using Intestinal-origin Probiotics Strains
(Widodo, Wahyuningsih, Tutik Dwi, Nurrochmad, Arief, Harsita, Pradipta Ayu, and Anindita, Nosa Septiana)
Penulis/Author
Prof. Widodo, S.P., M.Sc. Ph.D. (1)
Tanggal/Date
2016
Kata Kunci/Keyword
Abstrak/Abstract
The objective of this study was to evaluate the ability of intestinal-origin probiotics strains in alleviating hyperglycemia and hypercholesterolemia in rats (Rattus norvegicus) in vivo. Five strains of Lactic Acid Bacteria consists of Lactobacillus casei strain AP and AH, and Pediococcus acidilactici strains AA, BE and BK were previously isolated and identified from faeces of infants who consumed breast milk as the only source of diet. In vitro evaluation those five strains showed their potential as probiotic based on their capability to grow on media pH 2.0, with 1.5% concentration of bile salts, the ability to attach on gastric mucin in vitro, and their ability to inhibit the growth of pathogen. Evaluation on the ability to use prebiotic inulin as carbon source showed that Lactobacillus casei (strain AP and AG) and Pediococcus acidilactici strain BE had the ability to degrade inulin as a prebiotic. Evaluation of probiotics on their capability to synthesize conjugated linoleic acid from free linoleic acid showed that only Lactobacillus casei strain AP was able to convert more than 60% of free linoleic acid to conjugated linoleic acid in the media. Further in vivo studies using rats (Rattus norvegicus) showed that Lactobacillus casei strain AP had the ability to alleviate hyperglycemia and hypercholesterolemia. The ability of Lactobacillus casei strain AP in reducing hyperglycemia was comparable with that of metformin (anti-hyperglycemia drug) provided orally at level 45 mg/kg of body weight.
