| Penulis/Author |
Dr. Ir. Sigit Bintara, M.Si., IPU., ASEAN Eng. (1) ; Prof. Ir. Ismaya, M.Sc., Ph.D. (2); Prof. Ir. Diah Tri Widayati, MP., Ph.D., IPM (3); Ir. Riyan Nugroho Aji, S.Pt., M.Sc., IPP (4); Widya Asmarawati, S.Pt., M.Sc. (5) |
| Abstrak/Abstract |
Semen processing can reduce the motility and viability of the sperm. This can be caused by an oxidative reaction that results in the increase of free radicals in the semen. The addition of antioxidants from noni (Morinda citrifolia Linn) extract is expected to overcome the presence of free radicals in semen. The focus of this research was to evaluate the shelf life of liquid semen which could still be used for insemination in thin tail sheep semen diluted with Egg Yolk Citrate and noni extract. Semen was collected from 2 years-old thin tail sheep using an artificial vagina. The sperm was analyzed at the UGM Faculty of Animal Science's Laboratory of Animal Physiology and Reproduction. In this research, the treatments were egg yolk citrate diluent (P0) as a control, egg yolk citrate diluent and 4% noni extract (P1), egg yolk citrate diluent and 6% noni extract (P2), and egg yolk citrate diluent and 8% noni extract (P3). The variables measured were the quality of the semen through macroscopic examination including volume, colour, odor, pH, and consistency, as well as microscopic examination including concentration, motility, viability, and abnormalities of spermatozoa. Data obtained from macroscopic examination were analyzed descriptively, whereas the microscopic study findings were statistically analysed using a Completely Randomized Design (CRD). The results showed that the addition of noni extract could improve maintaining (P<0.05) motility and viability of spermatozoa stored at 5oC. The motility of spermatozoa on the fifth day at P0, P1, P2, and P3 were 30.3%, 44.2%, 40.7% and 39.6%, respectively. The viability of spermatozoa on the fifth day at P0, P1, P2, and P3 were 55.4%, 68.4%, 64.3% and 64.4%, respectively. The motility of spermatozoa on the sixth day at P0, P1, P2, and P3 were 25.2%, 35.3%, 31.6% and 30.3%, respectively. The viability of spermatozoa on the sixth day at P0, P1, P2, and P3 were 40.3%, 47.2%, 45.4% and 45.2%, respectively. The addition of 4% noni fruit extract generated the best results in terms of preserving motility (44.2%) and spermatozoa viability (68.4%) after 5 days of storage. The conclusion of this study, day five storage of thin tail sheep liquid semen diluted with egg yolk citrate and 4% noni fruit extract at 5oC was still eligible for insemination. |
