Stage Specificity of Eurycomanone Isolated from Eurycoma longifolia on Plasmodium falciparum Cycles
Penulis/Author
Prof Dr.dr. Eti Nurwening Sholikhah, M.Kes. (1); Dr. dr. Mahardika Agus Wijayanti, DTM&H., M.Kes. (2); Prof. Dr. apt. Ratna Asmah Susidarti, MS. (3); Dr. apt. Indah Purwantini, S.Si., M.Si. (4); Prof. Dr. Mustofa, Apt., M.Kes. (7)
Tanggal/Date
2016
Kata Kunci/Keyword
Abstrak/Abstract
Eurycomanone is the most active compounds in the roots of
Eurycoma longifolia and shown to have in vitro antiplasmodial activity.
However, the stage of Plasmodium falciparum cycles which are sensitive to
eurycomanone have not been investigated. This study was conducted to
investigate stage specificity of eurycomanone at various stages of P.
falciparum life cycles. Stage specificity of eurycomanone at various stages
of P. falciparum was performed on P. falciparum culture in vitro. A total of
100 µL of solution containing P. falciparum at ring stage after
synchronized with 1-2% parasitemia (hematocrit 3%) were included in 96
wells microcultures and then added 100 µL of solution containing
eurycomanone with 6 various concentrations. The specificity of
eurycomanone was evaluated microscopically by counting the percentage
of each stage of P. falciparum after for 8, 16, 24, 32, 40, 48, 56, 64 and 72
h incubation time, compared with control without any compound. The
results showed that eurycomanone can kill ring stage of P. falciparum and
may inhibit the development of young schizont to mature schizont in vitro.
However, it needs further investigations for the mechanism.