| Penulis/Author |
Dr. Ir. Sigit Bintara, M.Si., IPU., ASEAN Eng. (1) ; Prof. Ir. Dyah Maharani, S.Pt., M.P., Ph.D., IPM (2); Prof. Ir. I Gede Suparta Budisatria, M.Sc., Ph.D., IPU., ASEAN Eng. (3); Prof. Dr. Ir. Jafendi Hasoloan Purba Sidadolog (4); Prof. Dr. Ir. Sumadi, M.S., IPM (5); Prof. Dr. Ir. Lies Mira Yusiati, SU., IPU., ASEAN Eng (6); I Made Londra (7); Ir. Riyan Nugroho Aji, S.Pt., M.Sc., IPP (8) |
| Abstrak/Abstract |
This research aims to study the sperm characteristics of Gembrong goat
in Bali before and after freezing, using citrate-egg yolk (CEY) and tris-egg yolk
(TEY) diluents. The sperm of six goats was collected using an artifiial vagina. The
results showed that there were no signifiant differences between the use of CEY
and TEY diluents on motility, viability, and abnormalities of spermatozoa neither
before nor after freezing. Before freezing, motility was CEY 85 ± 3.5, TEY 85 ± 2.1;
viability was CEY 90 ± 3.5, TEY 92 ± 2.1; and abnormalities were CEY 11 ± 3.5,
TEY 10 ± 2, 1, while after freezing, motility was CEY 45 ± 3.5 b, TEY 46 ± 4.1;
viability was CEY 70 ± 3.5, TEY 72 ± 4.1; and abnormalities were CEY 15 ± 3.5,
TEY 16 ± 4.1. However, the freezing process signifiantly (p < 0.01) lowered sperm
quality in terms of motility, viability, and abnormalities, both for CEY and TEY
diluents. In CEY diluent, motility before was 85 ± 3.5 and after was 45 ± 3.5, viability before was 90 ± 3.5 and after was 70 ± 3.5, and abnormalities before were
11 ± 3.5 and after were 15 ± 3.5. In TEY diluent, motility before was 85 ± 2.1 and
after was 46 ± 4.1, viability before was 92 ± 2.1 and after was 72 ± 4.1, and abnormalities before were 10 ± 2.1 and after were 16 ± 4.1. Dilution and freezing of
Gembrong goat sperm using citrate-egg yolk and tris-egg yolk showed no signifcant differences, but the freezing process caused a decline in sperm quality. |
