Isolation and selection of proteolytic lactic acid bacteria from colostrum of dairy cattle
Penulis/Author
ANGGA RIZKI RAMADHAN (1); Prof. Dr. Ir. Zaenal Bachruddin, M.Sc., IPU., ASEAN Eng. (2); Prof. Widodo, S.P., M.Sc. Ph.D. (3); Prof. Ir. Yuny Erwanto, S.Pt., M.P., Ph.D., IPM (4); Dr. Ir. Chusnul Hanim, M.Si., IPM., ASEAN Eng. (5)
Tanggal/Date
28 2021
Kata Kunci/Keyword
Abstrak/Abstract
Lactic Acid Bacteria (LAB) are bacteria that produce lactic acid and are very useful
bacteria, one of which can act as a probiotic because it can produce bacteriocins and produce
peptide compounds with high biological activity. Colostrum is the first fluid that comes out of
the udder of the mother cattle after giving birth which is very good for newborns because it
contains antibodies. This research aimed to isolate, selection and identify the types of LAB
found in dairy cow colostrum, especially LAB which have superior proteolytic activity. LAB
obtained is expected to have a positive effect on livestock productivity. This research was
conducted In-vitro in a laboratory. The research procedure included the isolation of LAB from
the colostrum of dairy cows, then the selection procedure by looking at the best protease
enzyme activity qualitatively and quantitatively, and testing for resistance to low pH. Lactic
acid isolation, selection, and identification were analyzed using descriptive analysis and
quantitative analysis using one-way ANOVA for a completely randomized design. The results
obtained 6 superior proteolytic LAB isolates based on the protease enzyme activity coded LAB
pro 4, LAB pro 3, LAB pro 8, LAB pro 9, LAB pro 21, and LAB pro 20
