Screening of alpha-glucosidase inhibitor-producing lactic acid bacteria from Ganoderma lucidum
Penulis/Author
Prof. Dr. Endah Retnaningrum, S.Si., M.Eng. (4); Prof. Drs. Langkah Sembiring, M.Sc., Ph.D. (5)
Tanggal/Date
2015
Kata Kunci/Keyword
Abstrak/Abstract
Ganoderma lucidum has widely known as herbal medicine for diabetes. Recently, alphaglucosidase
inhibitors (AGI) have also been reported for their ability to reduce blood sugar level in type 2 diabetes. The
enzyme inhibitors can be derived from bacteria that live in Ganoderma lucidum as their host. The aims of this
study were to isolate and conduct AGI-producing endophytic lactic acid bacteria screening from Ganoderma
lucidum. Fourteen isolates were identified as lactic acid bacteria. The crude extracts were examined to measure
their alphaglucosidase inhibition activity. The results showed that the crude extract from L17 isolate had the
highest AGI activity out of 14 isolates. According to Line-Weaverberg plot, L17 crude extract was competitive
inhibitor and identified as Lactococcus sp. In conclusion, L17 isolate from Ganoderma lucidum has potential to
produce AGI that could be used as functional food alternative for diabetes
