Abstrak/Abstract |
Among many species in the genus Tobamovirus, Kyuri green mottle
mosaic virus (KGMMV) is known as a major seed-borne virus
infecting cucurbit plants, including melon, which possesses no known
resistance. The identification of genetic resistance to KGMMV in
Indonesia (KGMMV-YM) in melons is of economic importance.
Thus, 45 melon cultigens collected from 17 Asian countries were
screened by manual virus inoculation and resistance to KGMMV-YM
was examined by visual observation, enzyme-linked immunosorbent
assay and reverse transcription-polymerase chain reaction (RT-PCR).
The results show that resistance to KGMMV-YM was found in
Mawatauri, Kohimeuri, PI 161375 and PI 371795, and analysis by
RT-PCR showed the presence of KGMMV-YM in susceptible
cultigens as early as 3 days after leaf inoculation |