| Abstrak/Abstract |
Sugarcane (Saccharum officinarum L.) is one of the main crops in Indonesia and is
known as a sugar-producing plant. Sugar belongs to one of the 9 essential culinary ingredients
in Indonesia. With the high demand for sugar the government in 2017 imported 109,147 tons
of sugar or equivalent to IDR 823.8 billion to fulfill the demand. Efforts to increase sugarcane
productivity are often constrained by complex pests that attack sugarcane shoots. One way to
overcome it is to breed plant varieties that are resistant and of good quality. The purposes of
the present study were to screen sugarcane shoot borer resistance gene using the molecular
marker SacMPI-Like 3, to determine the phylogenetic relationship between MPI in sugarcane
with other species in the family Andropogenae, and to determine the most effective molecular
markers for detecting MPI. The primer was designed by using Primer3 Plus. DNA samples of
22 sugarcane varieties derived from the Genetics and Breeding Laboratory, Biology Faculty of
Gadjah Mada University were PCR-amplified using 3 selected primer designs. Results showed
that all of the 22 samples of sugarcane DNA contained SacMPI-Like 3 ranged from 500 to 600
bp. Some of the samples were sequenced to obtain the arrangement of nucleotide bases for
comparison with samples from the NCBI GeneBank. The phylogenetic tree analysis showed
that 3 large clusters were formed depended on the presence of MPI. The first cluster consisted
of SacMPI1_PS_58, SacMPI1_PS_951, SacMPI2_PS_384, SacMPI2_PS_951,
SacMPI3_PS_851, SacMPI3_PS_951, CA282462.1_SHC (Saccharum officinarum L.),
XR002447315.1_ SB, XR002447314.1_SB (Sorghum bicolor), XR003227569.1 PH,
XR003227568.1_PH (Panicum hallii) EU972719.1_ZM, NM001158424.2_ZM,
EU960955.1_ZM, and EU951995.1_ZM (Zea mays). The second cluster consisted of
XM024458314.1_BD (Brachypodium distachyon), CT827984.1_OZ, and
XM026021872.1_OZ (Oryza sativa). |
