ScanningElectronMicroscopyAnalysis of Tea’s Embryo Axis Explant Cultured on Murashige and Skoog Medium Containing 2,4-Dichlorophenoxyacetic acid
Penulis/Author
RATNA DEWI ESKUNDARI (1); Prof. Dr. Ir. Taryono, M.Sc. (2); Prof. Dr. Ir. Didik Indradewa, Dip.Agr.St. (3); Prof. Dr. Yekti Asih Purwestri, S.Si., M.Si. (4)
Tanggal/Date
12 2024
Kata Kunci/Keyword
Abstrak/Abstract
Camellia sinensis L. is an important crop in Indonesia as healthy beverage that
contains several secondary metabolism compounds, such as polyphenols and
catechins. Tissue culture including somatic embryogenesis and organogenesis
has been used for propagating plant for various needs. In this present shortcommunication, scanning electron microscopic (SEM) analysis of tea was conducted and discussed. This study aimed to investigate surface ultrastructure
of TRI2025 embryo axis tea clone cultured on Murashige and Skoog (MS) medium containing 2,4-Dichlorophenoxyacetic acid (2,4-D). The results revealed
two different forms of explant’s development, i.e. somatic embryo and
transitional form between somatic embryogenesis and organogenesis;
or called by “Globular-like Structure” (GLS). Surface ultrastructure
analysis of somatic embryo and GLS revealed respectively many
stages of somatic embryo development i.e. globular, torpedo, and cotyledon
stage, and leaf development form GLS regeneration.
