RP-HPLC-UV validation method for levofloxacin hemihydrate estimation in the nano polymeric ocular preparation
Penulis/Author
INDRI MAHARINI (1); Dr.rer.nat. Ronny Martien, M.Si. (2); Prof. Dr. apt. Akhmad Kharis Nugroho, S.Si., M.Si. (3); dr. Supanji, Sp.M(K), M.Kes., Ph.D. (4); Dr. Adhyatmika, M.Biotech., Apt. (5)
Tanggal/Date
2022
Kata Kunci/Keyword
Abstrak/Abstract
The specific and accurate reversed-phase HPLC-UV method has been validated to determine levofloxacin hemihydrate (LEVH) level. The separation was conducted at C 18 analytical column by administering mobile phase acetonitrile, methanol, and phosphate buffer (pH 3) with the ratio of 17:3:80. The flow rate of the mobile phase was 1 mL/min with a UV detector at 295 nm wavelength. Analytical methods validation evaluated includes specificity, linearity, accuracy, precision, LOD, LOQ, and robustness. The implementation of the analytical method was employed to determine LEVH level in ocular polymeric nanoparticles preparations. The test was specific for LEVH with the retention time of 7.66 min. Linearity was obtained from the concentration range of 4.8 µg/mL to 29.04 µg/mL. All method validation criteria are within the acceptable range. The developed method can be applied for LEVH polymeric nano-formulation analysis.
Rumpun Ilmu
Hidrometeorologi Departemen Geografi Pembangunan
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
1-s2_0-S1878535221005979-main.pdf
[PAK] Full Dokumen
2
Turnitin RP-HPLC-UV validation method for levofloxacin hemihydrate estimation in the nano polymeric ocular preparation.pdf
[PAK] Cek Similarity
3
L1 Penghargaan Karya Ilmiah Telah Terbit Levofloxacin.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
4
5_ Surat Pernyataan Tanpa EC RP-HPLC-UV - dr_ Supanji.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
5
RP-HPLC-UV validation method for levofloxacin hemihydrate estimation in the nano polymeric ocular preparation.pdf
