Karya
Judul/Title Resistance Response of Chilli (Capsicum annuum L.) F1 to Fusarium oxysporum Involves Expression of the CaChi2 Gene
Penulis/Author Rejeki Siti Ferniah (1) ; Prof. Rina Sri Kasiamdari, S.Si., Ph.D. (2); Prof. Ir. Achmadi Priyatmojo, M.Sc., Ph.D. (3); Prof. Dr. Budi S. Daryono, M.Agr.Sc. (4)
Tanggal/Date 6 2018
Kata Kunci/Keyword
Abstrak/Abstract Abstract: Cross-breeding is a method of producing progeny with better resistance to pathogens. Resistance to pathogens usually involves pathogenesis-related (PR) proteins. Class II chitinase is an example of a defensive PR protein in plants. The class II chitinase in chilli is coded by the CaChi2 gene. In this study, we crossed susceptible with resistant chilli cultivars, analysed the F1 resistance response against pathogenic F. oxysporum, and analysed the level of CaChi2 gene expression in the F1. Data were collected using disease severity index (DSI) determination and gene expression analysis by qRT-PCR (quantitative Reverse Transcriptase Polymerase Chain Reaction). Results showed that the DSI of F1 was not significantly different from the resistant ancestor. The relative CaChi2 expression level of F1 was higher than the susceptible ancestor but not significantly different from the resistant ancestor. We concluded that the F1 can be categorised as resistant to F. oxysporum, and the CaChi2 gene is involved in the molecular defense response. Keywords: Chitinase, Cross-Breeding, Disease Severity Index (DSI), qRT-PCR
Rumpun Ilmu Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
12018 Trop Life Sciences Res Rejeki-Rina-Budi vol 29-1 hal 29-37.pdf[PAK] Full Dokumen
2Resistance Response of Chilli (Capsicum annuum L) F1 to Fusarium oxysporum Involves Expression of the CaChi2 Gene.pdf[PAK] Cek Similarity
3PAK Rina SK_Full_2018 Resistance Response of Chilli (Capsicum annuum L) F1 to Fusarium oxysporum involves expression of the CaChi2 Gene.pdf[PAK] Full Dokumen
4PAK Rina SK_Sim_2018 Resistance Response of Chilli (Capsicum annuum L) F1 to Fusarium oxysporum involves expression of the CaChi2 Gene.pdf[PAK] Cek Similarity
52018 Surat Keterangan Publikasi Trop_Life_Sci_Res__Resistance Response of Chilli (Capsicum annuum L_) F1 ___Rejeki Siti Ferniah et al_.pdfDokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)