| Abstrak/Abstract |
Oleaginous yeasts are capable of accumulating high lipid concentration up to 20% of dry cell weight. High lipid content, a shorter life cycle, and similar a fatty acid composition to vegetable oils makes oleaginous yeast a potential lipid producer. Lipomyces maratuensis InaCC Y720 is a novel species isolated from Maratua Island, East Kalimantan, which has been reported as a potential yeast lipid producer. However, lipid productivity of the yeast is needed to be increased to make it suitable for an industrial scale. The aim of this study is to obtain potential mutant strains for the biodiesel industry. Random mutagenesis was applied by using commercial UV-C lamp on the strain which resulting in an 80?ath rate after three hours irradiation. Subsequent treatment was carried out using cerulenin as a selection agent for mutans, yielding six mutant strains. Among these strains, mutant 1 produced the highest lipid production, with a lipid concentration of 0.072 g/L and a lipid percentage of 8.603%. Nevertheless, when compared to the wild type, the lipid productivity of mutant 1 is low. Based on these results, the mutagenesis approach using commercial lamp UV-C has not obtained the expected mutants, so it is recommended to use different methods for future study. |
