| Penulis/Author |
Devy Pramudyah Wardani, S.Si., M.Sc. (1) ; Muhammad Arifin, S.Si., M.Sc., Ph.D. (2); Prof. Dr.Eng. Edi Suharyadi, S.Si., M.Eng. (3) ; Prof. Dr. Kamsul Abraha (4) |
| Abstrak/Abstract |
Gelatin is a biopolymer derived from collagen that is widely used in food and pharmaceutical products. Due to some
religion restrictions and health issues regarding the gelatin consumption which is extracted from certain species, it is
necessary to establish a robust, reliable, sensitive and simple quantitative method to detect gelatin from different parent
collagen species. To the best of our knowledge, there has not been a gelatin differentiation method based on optical
sensor that could detect gelatin from different species quantitatively. Surface plasmon resonance (SPR) based biosensor
is known to be a sensitive, simple and label free optical method for detecting biomaterials that is able to do quantitative
detection. Therefore, we have utilized SPR-based biosensor to detect the differentiation between bovine and porcine
gelatin in various concentration, from 0% to 10% (w/w). Here, we report the ability of SPR-based biosensor to detect
difference between both gelatins, its sensitivity toward the gelatin concentration change, its reliability and limit of
detection (LOD) and limit of quantification (LOQ) of the sensor. The sensor’s LOD and LOQ towards bovine gelatin
concentration are 0.38% and 1.26% (w/w), while towards porcine gelatin concentration are 0.66% and 2.20% (w/w),
respectively. The results show that SPR-based biosensor is a promising tool for detecting gelatin from different raw
materials quantitatively. |
