Our previous studies showed that kaffir lime leaf has cytotoxic effect on cervical cancer and
neuroblastoma. This was caused by action of bioactive compounds in extract. Bioactive compounds may cause
change in metabolism which can be detected by protein profile. Furthermore, dose and exposure duration possibly
affect the efficacy of chemoprevention agents. The objective of this study was to determine protein profile of T47D
and vero cell after being treated with kaffir lime leaf. We used different exposure duration which was 12 h, 24 h and
48 h. Dose of ethyl acetate extract and chloroform extract were 283 ?g · mL–1 and 129 ?g · mL–1
respectively,
whereas doxorubicin at a dose of 2.5 ?g · mL–1
and 5 ?g · mL–1 was used as reference. The extracted proteins were
separated with SDS?PAGE. The molecular weight of protein was determined by linear regression equation of log
protein marker molecule weight to the value of Rf (retention factor). Results showed that difference solvent in
extraction yields different bioactive compounds which led to different protein profiles in T47D and vero. Different
protein profile possibly caused by change of cell metabolism. T47D protein profile with ethyl acetate extract
treatment was inversely proportional to exposure duration. While, T47D under chloroform treatment had steady
protein profile without being affected by exposure duration. Furthermore, longer exposure duration caused particular
protein bands thicker and diminished in both extracts. The results warrant the further investigation of kaffir lime leaf
extracts as chemoprevention agents with less side effect.
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
kaffir lime_compressed.pdf
2
13_dok_Protein Profile.pdf
[PAK] Full Dokumen
3
13_similaritas_Protein profile of breast cancer cell line (T47D) with kaffir lime (Citrus hystrix DC_) leaf extract treatment.pdf
Cek Similarity
4
13_bukti korespondensi AIP ICBS.pdf
[PAK] Bukti Korespondensi Penulis
5
Full Dokumen Prosiding.pdf
[PAK] Full Dokumen
6
cek simil prosiding.pdf
Cek Similarity
7
Protein profile of breast cancer cell line-Prosiding.pdf
