| Abstrak/Abstract |
Rice (Oryza sativa L.) is an important crop for tropical countries, including Indonesia.
Central Bureau of Statistics Republic of Indonesia (2017) has explained that the rice production
has not fulfilled Indonesian market demand. Rice imports in 2016 increased in1.4 million tons.
Therefore, rice plant disease control should be applied. Tungro is known as “plant cancer” disease
of rice that is capable to damage 97% of the plant organ. Thus, the purpose of this study were to
determine the percentage of Rice tungro spherical virus (RTSV) attacks, the level of RTSV
symptoms and detection of RTSV-resistant genes in a population of rice fields in the Province of
Yogyakarta (DIY) using molecular marker design of Simple Sequence Repeat (SSR). The
samples used in this research were 60 samples obtained from five regencies in the province such
as Bantul, Gunungkidul, Kulon Progo, Sleman and Yogyakarta. DNA amplification was performed
with Rice Microsateliteprimers (RM) 10, RM336, and RM455. The results showed that the
percentage of RTSV attack in the district of Bantul, Gunungkidul Kulon Progo, Sleman and
Yogyakarta were respectively 1.12; 1.44; 1.17; 1.65; 0.96%; the level RTSV symptoms on rice
fields population in DIY was moderate to susceptible (4-7), molecular detection using SSR primer
RM10, RM336, RM455 which forms the target gene band consecutively 131 bp, 151 bp and 159
bp indicates that the adrift resistance genes in rice plants in rice field population Yogyakarta were
not found. This basic research was expected to be referred to as a means of scientific information
to find the best RTSV resistant rice varieties transformant.
Keywords: Oryza sativa L., Rice tungro spherical virus, Simple Sequence Repeat, Rice
Microsatelite
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