Potential of Ribosome-inactivating proteins (RIPs) of Mirabilis jalapa L. as an antiacne: Effect on Proliferation of Cultured Sebocyte Cells and its Antibacterial Activities against Propionibacterium acnes and Staphylococcus epidermidis
Penulis/Author
apt. Rumiyati, S.Si., M.Si., Ph.D. (1); Prof. Dr. apt. Sismindari, S.U. (2); Prof. Dr.rer.nat. apt. Raden Rara Endang Lukitaningsih, S.Si., M.Si. (3); Tri Yuliati, S.KM. (4)
Tanggal/Date
2014
Abstrak/Abstract
Previous researches showed that Ribosome-inactivating proteins (RIPs) isolated from Mirabilis jalapa L. demonstrated some bioactivities such as in vitro cytotoxic on cancer cell lines, antiinflamation and inhibition of nodule growth in animal model. Therefore, this protein has a potential to be developed as antiacne. Pathogenesis of acne is caused by some factors such as growth of sebocyte cells that produce sebum and infection caused by bacteria of Propionibacterium acnes and Staphylococcus epidermidis. This research was aimed to determine whether the extract has an effect on proliferation of primary cultured sebocyte cells and whether the extract possess antibacterial activities against Propionibacterium acnes and Staphylococcus epidermidis. Result of this study could have a benefit in the further development of use of protein of M. jalapa as antiacne. RIPs of M. jalapa leaves were extracted using phosphate buffer. This protein fraction was then determined in its activity on cleaving of supercoiled double stranded plasmid DNA in order to identify presence of RIPs in the extracts. The protein fraction was then tested on cultured sebocyte cells and on its antibacterial activity against Propionibacterium acnes and Staphylococcus epidermidis. Results demonstrated that the protein extract at concentration of 0.3-2.5 mg/mL has inhibition activity on proliferation of cultured sebocyte cells. The protein extract has bacteriostatic effect on growth of Staphylococcus epidermidis (MIC 10 mg/mL).
Rumpun Ilmu
Analisis Farmasi dan Kimia Medisinal
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Editorial Team IJPC.pdf
[PAK] Halaman Editorial
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International Journal of Pharmaceutical Chemistry _ Index Copernicus Journals Master List.pdf
[PAK] Halaman Cover
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Vol 4, No 4 (2014)-daftar isi.pdf
[PAK] Daftar Isi
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8_ Naskah Intl J_Pharm Chem Vol 4 No 4, 2014, p 130-133.pdf
[PAK] Full Dokumen
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Potential of Ribosome-inactivating proteins (RIPs) of Mirabilis jalapa L_ as an antiacne_ Effect on Proliferation of CulturedSebocyte Cells and Its Antibacterial Activities Against Propionibacterium a
