| Abstrak/Abstract |
Abstract
Gynura procumbens (Lour.) Merr. or known as Sambung Nyawa is one among of tradicional medicine that has been used by the Indonesian people to cure some diseases. Sambung Nyawa leaf has been widely studied the biological activity especially related with the chemoprevention effect. Sambung Nyawa leaf is known to be contained flavonoid compounds, unsaturated sterols, triterpenes, polyphenols and essential oils. The present study aimed to evaluate the cytotoxicity activity of some fractions (water, ethyl acetate, hexane and hexane-insoluble fractions) of Gynura procumbens ethanolic extract.
The cytotoxic effect of four fractions were examined using MTT assay and was performed on four types of cell line namely HeLa, T47D, WiDR, and Vero cells. The results showed that the water fraction had a very weak cytotoxic activity on the fourth of cell types, while the other fractions have varies activity in the fourth cell lines. The hexane fraction showed the highest activity towards Vero cells with an IC-50 value about 400 ?g / mL. On the other hand, the hexane fraction had the highest activity with the IC-50 of 200 ?g / mL towards HeLa cells. Ethyl acetate, hexane and the hexane-insoluble fractions presented a similar activity on T47D cells. While on WiDR cells, the activity of all fractions were very weak.
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