| Abstrak/Abstract |
Objective. The aim of this study is to analyze the effect of grape seed extract containing cyanidin on osteoclastogenesis (by means of receptor activator of nuclear factor-? B ligand/RANKL and osteoprotegerin/OPG levels) and the number of osteoclast during orthodontic relapse in wistar rats. Materials and Methods. This study is an in vivo quasi experimental research. A total of 32 male wistar rats were used in the study, which were randomly split equally into two groups, grape seed (GS) and control group (CG). All rats were given an orthodontic force of 35 cN using a stainless steel 3-spin coil spring that activated for 7 days and then conditioned to be passive. During this phase, GS group was administered grape seed extract containing cyanidin once per day. Orthodontic appliances were removed from both groups afterward, and then the alveolar bone tissue was isolated consecutively according to observation days (days 1, 3, 7, and 14), while OPG and RANKL levels were analyzed in their gingival crevicular fluid using a specific enzyme-linked immunosorbent assay (ELISA). Tissues were then stained with hematoxylin–eosin and observed under a light microscope to count the number of osteoclast cells. Data were analyzed statistically using an independent t-test (p<0.05). Results. The number of osteoclast in the GS group was significantly lower than that in the CG group on all experiment days (p=0.021;p=0.001;p=0.024;p=0.001;p<0.05). ELISA results showed that the RANKL level of the GS group was significantly lower on days 3 and 7 (p=0.025;p=0.039;p<0.05), while the OPG level was significantly higher on days 1 and 3 in the GS group than in the CG group (p=0.039;p=0.021;p<0.05). Conclusion. Grape seed extract can prevent post-orthodontic relapse movement by inhibiting osteoclastogenesis and reducing the number of osteoclast in wistar rats.
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