| Abstrak/Abstract |
In this work, bioceramic carbonated hydroxyapatite (CHA) was synthesized from abalone mussel shells (Halioitis
asinina) using a co-precipitation method; CHA-based scaffolds were fabricated with honeycomb (HCB) as the
porogen agent. The concentration of HCB porogen varied among 10, 20, 30, and 40 wt%. The Energy Dispersive
X-Ray Spectroscopy (EDS) analysis revealed that the Ca/P molar ratio of CHA was 1.73, which was close to
natural bone’s Ca/P molar ratio of 1.71. Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Diffractometer (XRD) tests revealed that the formed phase of the synthesized CHA was of the B-type. Analysis of the
pores structure showed an increasing porogen concentration, pores size, and porosity of the scaffold. The
addition of HCB porogen also decreased the crystallite size. This was very good for bone growth because the low
crystallinity created dislocations, making it easier for cells to proliferate. Based on the results of cell viability
assay on scaffold CHA + HCB 40 wt%, the growth of MC3T3E1 cells was inhibited beginning at a scaffold
concentration of 500 ?g/mL because the percentage of viability decreased to ~ 115 % and the IC50 value of
MC3T3E1 cells on the scaffold to 691830.97 ?g/mL. Based on to the one-way ANOVA, those result reflected no
statistically significant differences in the average of cell viability value in the five groups (p > 0.05). The cell
metabolic activity and morphology of the CHA + HCB 40 wt% scaffold enable it to facilitate the attachment of
MC3T3E1 cells on its surface. Thus, HCB 40 wt% was the best concentration to fabricate the scaffold based on the
criteria for pores structure, crystallographic properties, chemical decomposition process and cell viability for
biomedical applications. |
