| Abstrak/Abstract |
Gene markers are often usefull for selection process of cattle on commercial certain phenotypes.
Saanen goat has predominance in milk production traits. Milk fat content is one of the commercial traits
of milk production. Diacylglycerol Acyltransferase1 (DGAT1) gene was involved in the final process of
triglyceride synthesis and associated with milk fat content. Single nucleotide subtitution or insertiondeletion
in certain sequence of DGAT1 gene could be gene markers for milk fat content. The objective of
this study was to identify differences variation in milk fat content among individual Saanen goats, to
identify exon 14-16 polymorphism of DGAT1 gene and to examine its correlation between
polymorphism and milk fat content on Saanen goats. The methodologies was collection blood and milk
samples, DNA isolation from blood, amplification of DGAT1 genes, sequencing, detection of
polymorphism of exon 14-16 DGAT1 gene with Polymerase Chain Reaction-Single Strand
Polymorphism Identification of DGAT1 Gene in Saanen Goat (L. M. Y. D. Darmoatmodjo et al.) 211
Conformation Polymorphism (PCR-SSCP), and examination milk quality on three lactation months
using Lactoscan. The average of daily milk production from 70 Saanen goat was 2340±709 mL,
1880±632 mL, 1432±536 mL and average of milk fat content was 3.07±0.52%, 2.53±0.55%,
3.00±0.55% for the first, second and third months of lactation, respectively. The amplicon size of exon
14-16 of DGAT1 gene was ± 350 bp and showed monomorphic pattern from PCR-SSCP. Variation of
milk fat content on Saanen goats not caused by polymorphism of exon 14-16 DGAT1 gene. |
