| Penulis/Author |
Andina Setyawati, M.N (1); Prof. Dr. Mae Sri Hartati Wahyuningsih, Apt., M.Si. (2) ; dr. Dwi Aris Agung Nugrahaningsih, M.Sc., Ph.D (3); Prof. Dr. Christantie Effendy, S.Kp., M.Kes. (4); Firas Fneish (5); Gerhard Fortwengel (6) |
| Abstrak/Abstract |
Introduction: Piper crocatum Ruiz & Pav (P. crocatum) has been reported to accelerate the diabetic wound
healing process empirically. Some studies showed the benefits of P. crocatum in treating various diseases
but its mechanisms in diabetic wound healing have never been reported. In the present study we inves-
tigated the diabetic wound healing activity of the active fraction of P. crocatum on wounded hyper-
glycemia fibroblasts (wHFs).
Methods: Bioassay-guided fractionation was performed to get the most active fraction. The selected
active fraction was applied to wHFs within 72 h incubation. Mimicking a diabetic condition was done
using basal glucose media containing an additional 17 mMol/L D-glucose. A wound was simulated via
the scratch assay. The collagen deposition was measured using Picro-Sirius Red and wound closure
was measured using scratch wound assay. Underlying mechanisms through p53, aSMA, SOD1 and E-
cadherin were measured using western blotting.
Results: We reported that F IV is the most active fraction of P. crocatum. We confirmed that F IV\(7.81 mg/ml,
15.62 mg/ml, 31.25 mg/ml, 62.5 mg/ml, and 125 mg/ml) induced the collagen deposition and wound closure
of wHFs. Furthermore, F IV treatment (7.81 mg/ml, 15.62 mg/ml, 31.25 mg/ml) down-regulated the protein
expression level of p53 and up-regulated the protein expression levels of aSMA, E-cadherin, and SOD1.
Discussion/conclusions: Our findings suggest that ameliorating collagen deposition and wound closure
through protein regulation of p53, aSMA, E-cadherin, and SOD1 are some of the mechanisms by which
FIV of P. crocatum is involved in diabetic wound healing therapy.
Ó 2021 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access
article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
