Karya
Judul/Title PHYLOGENETIC ANALYSIS OF CULEX TRITAENIORHYNCHUS AND CULEX VISHNUI VECTOR OF JAPANESE ENCEPHALITIS VIRUS
Penulis/Author Dr. Dra. Raden Roro Upiek Ngesti Wibawaning Astuti, B.Sc., DAP&E. M.Biomed. (1) ; Dr. Budi Mulyaningsih, Apt., M.S (2); Dr. R.C. Hidayat Soesilohadi, M.S. (3); Prof. Dr. drh. Raden Wisnu Nurcahyo (4); Prof. Dr. Suwarno Hadisusanto, S.U. (5)
Tanggal/Date 2019
Kata Kunci/Keyword
Abstrak/Abstract Culex tritaeniorhynchus and Culex vishnui are medically important mosquitoes that transmit Japanese Encephalitis (JE) virus. There is less information about the recording data and research due to genetic character differences among them. The objective of this study was to examine the genetic variation of Cx. tritaeniorhynchus and Cx. vishnui in 3 site of Central Java using PCR-RAPD. The study was done from January to November 2017, in Pekalongan City, Pekalongan Regency and Semarang Regency. Adult female mosquitoes were collected by landing bite method. DNA of ten Cx. tritaeniorhynchus samples and fifteen samples of Cx. vishnui were purified using DNA kit extraction. Furthermore, PCR amplification was conducted with 5 RAPD primers (OPA 11, 12, 15, 16, and 20) and would run into 2% gel electrophoresis for 45 minutes. Cluster analysis was done by using MVSPTM software. The results showed 213 genetical characters of Cx. vishnui, while 142 characters were shown by Cx. tritaeniorhynchus. The dendograms showed 3 distinct grups of Cx. vishnui from 2 sites of Pekalongan and 1 site of Semarang, while Cx. tritaeniorhynchus showed 2 distinct grups, which were 1 group from Pekalongan and 1 group from Semarang. Low genetic similarity (<10%) was shown Cx. vishnui from Pekalongan City and Pekalongan District, and there was no genetic similarity in Cx. tritaeniorhynchus from Pekalongan and from Semarang. It is concluded that the polymorphism of Cx tritaeniorhynchus and Cx. vishnui reached 100%.
Rumpun Ilmu Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language English
Level Nasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
13_ Phylogenetic Analysis of Culex tritaeniorhynchus and Culex vishnui Vector of Japanese Encephalitis Virus.pdf[PAK] Full Dokumen
2Bukti Korespondensi Phylogenetic Analysis of Culex.pdf[PAK] Bukti Korespondensi Penulis