Karya
Judul/Title Phenotypes, Performance, and Insulin Gene (INS) Single Nucleotide Polymorphism (SNP) C1549T Genotyping of Indonesian Meat‐Type Chicken Breed
Penulis/Author DYAHAYU NISA ARINI (1); MAULANA DIAS PRATAMA (2); GILANG ILHAM FIRMANSYAH (3); I WAYAN SWARAUTAMA M (4); Ayudha Bahana Ilham Perdamaian (5); Prof. Dr. Budi S. Daryono, M.Agr.Sc. (6)
Tanggal/Date 31 2022
Kata Kunci/Keyword
Abstrak/Abstract Pelung chicken, as one of the Indonesian indigenous chicken breeds, is known for its distinct characteris-tics. Pelung chicken has been an object of selective breeding programs due to its slow-growing perform-ance, particularly in live weight gain. Through selective breeding, the backcross and its reciprocal genera-tions have been produced. This study was aimed to identify the phenotypes, performance in live weight gain, and insulin gene (INS) single-nucleotide polymorphism (SNP) C1549T genotyping. The phenotypes consisted of morphometrics and morphological traits. The tetra-primer amplification refractory mutation system polymerase chain reaction (T-ARMS-PCR) method was used to detect the SNP C1549T of the INS gene. The live weight gain of reciprocal backcross (RBC1) outperformed the first backcross (BC1) chick-ens. Morphometrics and morphological traits of BC1 and RBC1 indicated a directional selection effect to-wards pelung chicken. The transition mutation of SNP C1549T was only detected in RBC1 chickens as CT genotype (44.44%) and TT genotype (55.56%). The presence of SNP C1549T might have a significant as-sociation with the live weight gain of RBC1. T-ARMS-PCR method is suitable for the rapid detection of single nucleotide polymorphisms. Additional studies are required to confirm the association of INS gene polymorphism and live weight gain with a larger population size.
Rumpun Ilmu Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1IJAAS_Volume 12_Issue 2_Page 363-370.pdfBukti Published
2L1.pdfDokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
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