Pharmacokinetic Herb-Drug Interactions of Glipizide with Andrographis paniculata (Burm. f.) and Andrographolide in Normal and Diabetic Rats by Validated HPLC Method
Penulis/Author
ELZA SUNDHANI (1); Prof. Dr. apt. Agung Endro Nugroho, S.Si., M.Si. (2); Prof. Dr. apt. Arief Nurrochmad, M.Si., M.Sc. (3); Prof. Dr. Ika Puspita Sari, S.Si., M.Si., Apt. (4); Dita Amalia Prihati, S.T. (5); Prof. Dr.rer.nat. apt. Raden Rara Endang Lukitaningsih, S.Si., M.Si. (6)
Tanggal/Date
14 2022
Kata Kunci/Keyword
Abstrak/Abstract
Co-administered medicinal herbs can modify a drug’s pharmacokinetics (PK), effectiveness,
and toxicity. Andrographis paniculata (Burm. f.) ethanolic extract (APE) and andrographolide (AND)
(a potent CYP2C9 inducer/inhibitor) can alter the pharmacokinetic parameters of glipizide (GLZ).
This study aimed to determine the potential pharmacokinetics of herb–drug interactions between GLZ
and APE/AND in the plasma of normal and diabetic rats using the HPLC bioanalysis method. The
glipizide bioanalytical method established with RP-HPLC/UV instrument was validated following
the EMA guidelines. GLZ was administered alone and in combination with APE or AND to normal
and diabetic rats. The GLZ pharmacokinetic parameters were estimated according to the correlation
between concentration and sampling time using the PK solver program. A simple and rapid GLZ
bioanalysis technique with a lower limit of quantitation of 25 ng/mL was developed and presented
the following parameters: accuracy (error ≤ 15%), precision (CV ≤ 15%), selectivity, stability, and
linearity (R2 = 0.998) at concentrations ranging 25–1500 ng/mL. APE administration significantly
improved the Cmax and AUC0–t/AUC0–∞ GLZ values in normal and diabetic rats (p < 0.05). AND
significantly reduced the bioavailability of GLZ in diabetic rats with small values of T 1/2, Cmax,
and AUC0–t/AUC0–∞ (p < 0.05). This combination can be considered in administering medications
because it can influence the pharmacological effects of GLZ.
Rumpun Ilmu
Farmakologi dan Farmasi Klinik
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Elza EC.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
2
SJR Molecules.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
