| Abstrak/Abstract |
Phalaenopsis amabilis (L.) Blume is one of nature orchids with a high commercial value. The orchid cultivation is
difficult because the seeds are microscopic and don’t have the endosperm, so it needs to be done with in vitro
culture. This cultivation need medium that supports the growth of the orchids for example with the addition kind of
plant growth regulator that is auxin and cytokinin. This study was aimed determining effect of the plant growth
regulator of early development of P. amabilis orchid protocorm in vitro aspects of morphology and anatomy.Then
protocorm sub cultured on medium with NAA and TDZ combinations at various concentrations and incubated in a
room temperature. Protocorm orchid is then observed for 2 weeks which includes the percentage of growth,
percentage protocorm the forming buds and absorbing hair. Making the anatomical preparations made by taking
protocorm every week, stored in 70% alcohol solution and made preparations anatomy using Parafin method with
safranin staining. The results showed the addition of NAA and TDZ protocorm growth reached 100%. The protocorm
percentage of the forming buds as follows (0: 0, 0: 0.2, 0.5: 0, 0.5: 0.2, 1: 0 and 1: 0.2) ppm was 24%, 22%, 32%,
40%, 40% and 38% while the protocorm observed which form absorbing hair included 10%, 18%, 14%, 24%, 16%
and 0%. The cell diameter ranged from 39.2 ± 0.47?m, 0.97?m ± 44.4, 39.08 ± 0.5?m, 0.2?m ± 38.83, 39.5 ± 0.39?m
and 39.75 ± 0.28?m. Therefore we can conclude that the most optimal medium in P. amabilis orchid growing in vitro
is medium NP with a combination of NAA and TDZ (0.2: 0.5) ppm. |
