Dr. dr. Rul Afiyah Syarif, M.Kes. (1); Prof. Dr. Mae Sri Hartati Wahyuningsih, Apt., M.Si. (2); Prof. Dr. Mustofa, Apt., M.Kes. (3); Prof. dr. Ngatidjan M.Sc., Sp.FK(K) (4)
Tanggal/Date
1 2021
Kata Kunci/Keyword
Abstrak/Abstract
The previous research showed that 6th fraction (F6), an active fraction (F-akt), of Tithonia diversifolia (Hemsley) A.Gray leaves inhibited the Plasmodium growth. The growth of parasites requires energy obtained from the lactate dehydrogenase (LDH) activity. This study aimed to investigate the F6 (an active fraction) of T. diversifolia antiplasmodial activity on LDH levels of P. falciparum. The ring stage of P. falciparum D10 strain culture was divided into 3 groups, that were Plasmodium-uninfected erythrocytes (KTI), Plasmodium-infected erythrocytes (KI), and Plasmodium- infected erythrocytes treated by F-akt of T. diversifolia (KI+F-akt) 9.38-150 ?g/mL. The culture was incubated 48 h, the LDH in culture medium then was measured enzymatically. The difference of LDH level among groups was analyzed statistically using Anova. The LDH activity inhibition (IC50) was determined by probit analysis. The LDH level of KI group (362.33±133.18 U/L) was higher than KTI (270.33± 65.85 U/L) (p>0.05). LDH levels of KI+F-akt groups were lower than KI. The LDH level of P. falciparum treated by F-akt 9.38; 18.75; 37.50; and 150 ?g/mL were 365.5±129.5; 210.5±1.5; 195.5±81,5; and 111.5±53.5 U/L, respectively. There were no differences in LDH levels among study groups (p>0.05). F-akt of T. diversifolia inhibits LDH of P. falciparum strain D10 with IC50 value = 39.22 ?g/mL.
