Pengaruh Fucoidan terhadap Struktur Hepar Ikan Zebra (Danio Rerio, Hamilton 1822) yang Diberi Parasetamol Dosis Tinggi
Penulis/Author
Nur Indah Septriani, S.Si., M.Sc., Ph.D. (1); Eben Ezer Simanungkalit (2); Olvita Mayani (3); Indah Nur Fauziah (4); Desy Amelia Saputri (5); Anggi Rehulina Sitepu (6); Raafi Nur Ali (7); ASCARTI ADANINGGAR (8); Raden Roro Risang Ayu Dewayani Putri (9); Anita Restu Puji Raharjeng (10); Prof. Dr. Bambang Retnoaji, S.Si., M.Sc. (11); Dr. Ardaning Nuriliani, S.Si., M.Kes. (12); Dr.med.vet. drh. Hendry T.S.S.G. Saragih, M.P. (13); Zuliyati Rohmah, S.Si., M.Si., Ph.D.Eng. (14); Dr. Fajar Sofyantoro, S.Si., M.Sc. (15); Dr. Slamet Widiyanto, S.Si., M.Sc. (16); Dr.biol.hom. Nastiti Wijayanti, S.Si., M.Si. (17)
Tanggal/Date
2 2024
Kata Kunci/Keyword
Abstrak/Abstract
N-acetyl-p-aminophenol or paracetamol is an analgesic and antipyretics which can be obtained easily without a prescription. Consumption misuse and wrong dosage intake can lead to liver damage. Fucoidan has hepatoprotective activity that can stimulate hepatocyte regeneration. The aim of this study was to observe the histological liver structure of adult zebrafish that had been treated with paracetamol and fucoidan. The research design used was a completely randomized design (CRD) with zebrafish divided into four treatment groups; control (K) for 10 days, paracetamol 5 mM (P) for 3 days followed by RO water for 7 days, Paracetamol 5 mM for 3 days followed by fucoidan 100 g/mL (P + F 100) for 7 days and Paracetamol 5 mM for 3 days followed by fucoidan 500 g/mL (P + F 500) with replicates for each group of 6 fish. The study lasted for 10 days, data on liver histopathology was evaluated, fish body weight, swimming activity, and appetite was also evaluated. In all treatment groups, the results showed that the histological structure of the zebrafish liver experienced tissue damage in the form of hemorrhage, and cell damage in the form of vacuolization, pyknosis and necrosis. There was a decrease in swimming activity and appetite after paracetamol treatment, the group given fucoidan experienced a recovery in swimming activity and appetite. There was a significant difference (P <0.05) in liver damage between the control, paracetamol and the fucoidan treated groups. The P + F 100 and P + F 500 exhibited hepatocyte recovery after exposure to paracetamol. The P + F 500 group had better improvement than the P + F100 group. The result of this research showed that fucoidan can be used as a protective liver agent after paracetamol high dosage exposure.
