| Abstrak/Abstract |
Isolation of RNA or Ribonucleic Acid constitutes a basic technique in the field of molecular biology. RNA extraction was done to acquire pure mRNA where one of which can be used to see the expression of certain genes in an individual. Many methods can be used to perform RNA isolation, both conventional and modern methods. The purpose of this study were to compare the results of total RNA isolated using the CTAB3-LiCl (conventional) method and the Kit (modern) method and amlification target FaPYR1 and FaCHS genes in the ripening of strawberry fruits. RNA isolation was done using the Kit method referring to the Geneaid "RNA Total Mini Kit (Plant)" kit protocol. In general, the RNA isolation may consist of three stages: (1) Lysing the membrane or cell wall by physical and chemical destruction, (2) Removing unwanted components such as proteins, polysaccharides, and cell debris, (3) Searching for pure RNA by precipitation. The total RNA isolates were analyzed quantitatively by TECAN nanodrop spectrophotometer to determine the purity and concentration of RNA. The method of amplification is used Biorad thermal cylers. The results showed that the RNA isolated using the CTAB3-LiCl method had a higher RNA concentration than the Kit method, and the purity produced from the two methods did not differ significantly. Thus, total RNA isolated using the CTAB3-LiCl method is more optimal than the Kit method. The FaPYR1 and FaCHS genes amplificated in 627 bp, actin gene in 262 bp , and 26S-18S housekeeping gene in 146 bp to all samples selected. |
