| Abstrak/Abstract |
Lipase (triacylglycerol lipase, EC 3.1.1.3) is an enzyme capable of hydrolysing triacylglycerol to produce fatty acids and glycerol and reverse reaction of triacylglycerol synthesis from fatty acids and glycerol through transesterification. Lipase is an enzyme whose application is quite wide in the industrial sector, including detergent, paper, dairy, and food industries, as well as biodiesel synthesis. Lipases by yeasts have attracted industrial attention because of their fast production times and high stability. In a previous study, a lipase-producing yeast isolate was identified as Zygosaccharomyces mellis SG1.2 and had a productivity of 24.56 U/mg of biomass. This productivity value is quite potential to be a new source of lipase, besides Y. lypolitica which has been known as a lipase producer with a productivity of 0.758 U/mg. Lipase production by Zygosaccharomyces mellis SG1.2 needs to be increased by optimizing the production medium. The purposes of this study are to determine the significant component of the medium on lipase production and the optimum medium's ability to increase lipase production. Two steps of statistical optimization of production medium using Taguchi and RSM (Response Surface Methodology) methods. The data obtained will be analysed using Minitab 18 and SPSS 23 software. Most significant factors affecting lipase productivity are olive oil and peptone. The optimum medium composition consisted of 1.02% olive oil, 2.19% peptone, 0.05% MgSO4.7H2O, 0.05% KCl and 0.2% K2HPO4. Optimum medium was able to increase 1,8 folds of lipase productivity of Zygosaccharomyces mellis SG1.2 compared before optimization. |
