Optimization of HS‑SPME combined with GC–MS for key marker volatile organic compound analysis in Kappaphycus alvarezii with a chemometric approach
Penulis/Author
Diah Puspitasari, S.Pi., M.Sc. (1); Irene Punta Sanchez (2); Jose Luis P Calle (3); Dr. Widiastuti Setyaningsih, S.T.P., M.Sc. (4); Dr. Lideman, S.Pi., M.Sc. (5); Miguel Palma (6); Dr. Andriati Ningrum, S.T.P., M.Agr. (7); Dr. Manikharda, S.T.P., M.Agr. (8)
Tanggal/Date
5 2024
Kata Kunci/Keyword
Abstrak/Abstract
Kappaphycus alvarezii is one of the most essential edible seaweeds in the global trade of marine products. The product
quality will directly infuence the favor, odor, and impact on the human health of this edible seaweed. These functions are
associated with volatile organic compound (VOCs) content and are controlled by seaweed cultivation sites. This study investigated VOCs using HS-SPME combined with GC–MS and multivariate analysis to determine the VOCs to be used as key
markers for the quality of seaweeds from diferent cultivation sites. The HS-SPME factors, such as the amount of sample,
extraction temperature, and extraction time, were optimized based on Box–Behnken design (BBD) and response surface
methodology (RSM) for multi-response optimization (MRO). The precision of the approach varied from 1.17% (alkanes)
to 6.30% (carboxylic acids) for repeatability and 3.93% (furans) to 16.64% (sesquiterpenoids) for intermediate precision.
This method was applied to samples collected from nine diferent geographical regions in Indonesia. Principal Component
Analysis (PCA) was used to determine data distribution from 48 compounds in K. alvarezii from those diferent cultivation
sites. Based on PCA, four key marker VOCs, including heptadecane, 3-methylbutanoic acid, 2-pentyl furan, and 2-heptanone,
were successfully established.
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
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