| Abstrak/Abstract |
Green tea (Camellia sinensis L.) is known to have ability to protect skin against free radicals. This is
supported by polyphenol compound catechin. This research aims to determine the optimum HydrophilicLipophilic Balance (HLB) value of Tween 60 and Span 80 compositions on the optimum cream formula of
ethanol extract of green tea leaves. Tea leaves are extracted by macerating using 70% ethanol. Catechin in
extract is known from Thin Layer Chromatography (TLC) test with silica gel 60 F254 as stationary phase and
ethyl acetate:aquadest:formic acid (18:1:1 v/v) as mobile phase. Antioxidant activity is determined by 2,2 -Diphenyl-1-picryhydrazyl (DPPH) method and value of Inhibition Concentration 50% (IC50) is then
calculated. Formula optimization using Design Expert® version 7.1.5 (DX 7) software, Simplex Lattice
Design (SLD) method with two components Tween 60 and Span 80. Cream is characterized according to
physical properties organoleptic, homogeneity, viscosity, pH, spreadability, adhesiveness, and cream type.
The optimum formula obtained is then tested for physical stability for 4 weeks at room temperature
(28±2°C) and data are statistically analyzed using one-way ANOVA. The extract contains catechin proved
with Retention factor (Rf) value 0.8 and has antioxidant activity with IC50 value 56.35 ppm. 6.4% Tween 60
and 3.6% Span 80 result an optimum HLB value 11.1. It has viscosity 2897.50±35.94 mPa.s, spreadability
18.44±0.06 cm
2
, adhesiveness 0.85±0.05 seconds, and pH 4.530±0.002. Statistical test shows that the cream
is significantly altered at pH, but does not significantly change in viscosity, spreadability, and adhesiveness
after being stored for 4 weeks. |
