| Abstrak/Abstract |
Sambung Nyawa leaves
(
Gynura
p
rocumbens
(Lour.) Merr
has been widely used as herbal medi
cine
which requires a quality improvement of the dry extract for industrial production. Extraction solvent
optimization is one key factor which determines the quality. This research aims was to find out the optimal
ethanol
-
water composition as extraction s
olvent by using Simplex Lattice Design (SLD) method of which the
total phenolics, total flavonoids and DPPH radical scavenging activity were used as quality parameters.
Dried leaves as raw materials were pulverized and screened at Mesh 60, macerated (1:5)
with ethanol
-
water
compositition as 1:0; 0.7:0.3;
and 0.5:0.5v/v, shaked for 24
h, filtered. The procedure was repeated twice.
Filtrates were collected of which lactose were added (1:2)w/w and spray dried at 100
°C for 30
min. Dried
extracts yielded were eva
luated the quality by using SLD method of which the total phenolics, total flavonoids
as well as DPPH radical scavenging activity were used as parameters. Optimal SLD response was revealed at
the ethanol:w
ater composition of 0.66:0.34
-
0.75:0.25v/v (R
total
>
0.9). No significant difference of the above
mentioned parameters between the values resulted from the experiment and SLD formula. Correlation
analyses of total phenolics and total flavonoids towards DPPH
-
radical scavenging activity were found as
95.29% an
d 1.25%, respectively. |
