Optimization and validation of rp-hplc/uv detection for several compounds simultaneously in semi-purified extract of white tea
Penulis/Author
SHAUM SHIYAN (1); Prof. Dr.rer.nat. apt. Triana Hertiani, S.Si., M.Si. (2); Dr.rer.nat. Ronny Martien, M.Si. (3); Prof. Dr. apt. Akhmad Kharis Nugroho, S.Si., M.Si. (4)
Tanggal/Date
2019
Kata Kunci/Keyword
Abstrak/Abstract
Modification of separation, detection, and quantification of several compounds simultaneously in
white tea leaf extract using HPLC aims to obtain easy, fast, precise, and accurate analysis procedures. An HPLC optimization strategy was devised that allowed simultaneous determination of the effectiveness of
separation and quantification of gallic acid, theobromine, theophylline, catechin, caffeine and epigallocatechin gallate. The compounds were separated and isocratically on a LiChrocart-C18
column. The optimal conditions for separation were determined using central composite design (CCD)
with a solvent consisting of acetonitrile: methanol: 0.005% orthophosphoric acid (10:10:80 v/v/v)
at a 0.90 mL/min flow rate. Validation procedures for modified analysis methods have been carried
out following the terms and conditions. All validation points meet the requirements set by the
International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human
Use. The validation results were successfully applied to a white tea leaf extract. CCD is very effective for HPLC system optimization and was efficient for the simultaneous analysis of several compounds in white tea extract.
Rumpun Ilmu
Farmasetika dan Teknologi Farmasi
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
publikasi_Optimization_and_validation_of(1).pdf
Bukti Published
2
RSC_12_3_white tea full dokumen_compressed.pdf
[PAK] Full Dokumen
3
OPTIMIZATION AND VALIDATION OF RP-HPLC_UV DETECTION FOR SEVERAL COMPOUNDS SIMULTANEOUSLY IN SEMI-PURIFIED EXTRACT OF WHITE TEA.pdf
[PAK] Cek Similarity
4
Optimization and validation of rp hplc uv detection for several compounds simultaneously (1).pdf
