| Abstrak/Abstract |
Fusarium wilt of banana caused by Fusarium oxysporum
f. sp. cubense is widespread in Indonesia. However, the
distribution of tropical race 4 strains has not been well
studied. Thirty nine isolates of F. oxysporum f. sp.
cubense were collected from Java and 7 isolates were
from Sumatera, Bangka, and Kalimantan. All isolates
produced volatile odor when grown on steamed rice.
These isolates were further tested for their vegetative
compatibility with nitM testers of 20 reported vegetative
compatibility groups representing strains that belong to
race 1, 2, and 4. Three isolates formed heterokaryons
with nitM testers belong to race 1, 11 isolates with race
4, and the rest did not form heterokaryons with all nitM
testers used. F. oxysporum f. sp. cubense tropical race 4
specific primer pair was used to amplify a 1400 bp
fragment of tropical race 4 DNA. Seven isolates (Bnt2,
Mln1, Srg1, Bgl3, Bgl6, Lmp1, and Kjg1) produced the
1400 bp amplification product were therefore tropical
race 4. |
