Nuclear Magnetic Resonance Spectroscopy Based Metabolomic profiling of Tomato Fruit Long Shelf Life and Ripening
Penulis/Author
Enik Nurlaili Afifah, S.P., M.Sc. (1); Prof. Dr. Rudi Hari Murti, S.P., M.P. (2); Dr. Tri Rini Nuringtyas, S.Si., M.Sc. (3); Agus Budi Setiawan, S.P., M.Sc., Ph.D. (4)
Tanggal/Date
11 2021
Kata Kunci/Keyword
Abstrak/Abstract
Tomato has been classified into climacteric fruits that experience change in texture, aroma, firmness, and flavour during harvesting process. However, farmers and consumers prefer tomato fruits having a long shelf life. Tomato fruit ripening and long shelf life is genetically controlled and involves physiological and biochemical pathways. Therefore, the study of metabolomic profiling of ripening and non-ripening tomato is highly needed for developing biochemical markers in the breeding programs. Three tomato cultivars (Marta, GM2, and LA 175) were used in this study. 1H NMR spectroscopy (nuclear magnetic resonance) combined with multivariate data analysis was used to profiling the biochemical compounds of tomato shelf life. Quantitative data were analyzed using analysis of variance and then continued by Tukey’s HSD test at α = 0.05. Tomato LA 175 had a twice higher fruit shelf life compared to Marta (commercial tomato) and GM2 (non ripening tomato). However, GM2 was the higher of ethylene production compared to the other cultivars. Long shelf life tomato fruit is generally marked by a significant decreasing of fruit firmness, producing a high ethylene content, having a low of shelf life.