Nondestructive quantification of betalain secondary metabolites in red beet (Beta vulgaris) powder using near and mid infrared spectroscopy
Penulis/Author
Dr. Ir. Rudiati Evi Masithoh, S.T.P., M.Dev.Tech., IPU., Asean Eng. (1); Reza Adhitama Putra Hernanda (2); Hanim Zuhrotul Amanah, S.T.P., M.P., Ph.D. (3); Byoung-Kwan Cho (4); Prof. Dr. Abdul Rohman, S.F., M.Si., Apt. (5)
Tanggal/Date
1 2025
Kata Kunci/Keyword
Abstrak/Abstract
Betacyanin and betaxanthin are well-known secondary metabolites of betalains in red beet
(Beta vulgaris), which contribute to the natural red–purple and yellow–orange color. Various
analytical techniques, such as chromatography and nuclear magnetic resonance spectroscopy, are
currently available and have been performed to quantify the betalain content. However, evidence
shows that these methods remain arguable because of their disadvantages, such as being timeconsuming,
labor-intensive, destructive, and producing chemical wastes. This study aimed to
encompass the spectroscopic techniques through near-infrared (NIR) and mid-infrared (MIR)
spectroscopy coupled with partial least squares regression PLSR analysis for rapid and
nondestructive measurements of red beet powder betalain content. This study evaluated full and
effective wavebands to determine the optimum model for predicting betacyanin and betaxanthin
in red beet powder. The finding of effective wavebands was done by employing a variable
important in projection (VIP) technique. This study demonstrated that betacyanin and betaxanthin
were optimally predicted using NIR, with an R2 of 0.92 and a RMSEP of 0.07 mg/g for betacyanin
and R2 of 0.83 with RMSEP of 0.04 mg/g for betaxanthin. This optimum model was achieved
through the effective wavebands via VIP method. Thus, the proposed model is applicable for
predicting betalain content in red beet powder nondestructively.
