Nobiletin increased cytotoxic activity of doxorubicin on Mcf-7 cells but not on T47d cells
Penulis/Author
Prof. Dr. apt. Edy Meiyanto, M.Si. (1); Prof. Dr.rer.nat. apt. Adam Hermawan, S.Farm., M.Sc. (2); SENDY JUNEDI (3); ADITYA FITRIASARI (4); Prof. Dr. apt. Ratna Asmah Susidarti, MS. (5)
Tanggal/Date
2011
Kata Kunci/Keyword
Abstrak/Abstract
Nobiletin, a citrus flavonoid, shows strong cytotoxic effect in several
cancer cell lines. The aim of this research was to investigate cytotoxic
activities of nobiletin alone and in combination with doxorubicin. Cell
viability assay of nobiletin, doxorubicin, and combination treatments
were carried out by using MTT assay. Apoptosis assay was done using
double staining method using Ethidium Bromide-Acridine Orange. Cell
cycle distribution was determined by flowcytometry FACS-Calibur and
data was analyzed by using ModFit LT 3.0 program. Nobiletin (5, 10 and
15 ?M) increased cytotoxic effect of doxorubicin compared with
doxorubicin alone in MCF-7 cells but not in T47D cells. The strongest
cytotoxic activity was showed by the combination of 200 nM
doxorubicin and 15 ?M nobiletin in MCF-7 cells. Single treatment of
doxorubicin 200 nM, nobiletin 15 ?M and their combination induced
G2/M accumulation in MCF-7 cells. Combination of 15 ?M and
nobiletin 200 nM doxorubicin showed strong synergism on apoptosis
induction of MCF-7 cells. Single treatment of nobiletin 15 ?M and
doxorubicin 7.5 nM induced T47D cell accumulation in G1 phase and
G2/M phase respectively, while their combination induced accumulation
of T47D cells in G2/M phase. Nobiletin is potentially to be developed as
co-chemotherapeutic agent for breast cancer, while molecular mechanism
need to be explored
