Multiresponse optimization of a UPLC method for the simultaneous determination of tryptophan and 15 tryptophan-derived compounds using a Box-Behnken design with a desirability function
Penulis/Author
Dr. Widiastuti Setyaningsih, S.T.P., M.Sc. (1); Irfan Estiono Saputro (2); Ceferino A Carrera (3); Miguel Palma (4); Carmelo G Barroso (5)
Tanggal/Date
2017
Kata Kunci/Keyword
Abstrak/Abstract
A Box–Behnken design was used in conjunction with multiresponse optimization based on the desirabil- ity function to carry out the simultaneous separation of tryptophan and 15 derivatives by Ultra Performance Liquid Chromatography. The gradient composition of the mobile phase and the flow rate were optimized with respect to the resolution of severely overlapping chromatographic peaks and the total run time. Two different stationary phases were evaluated (hybrid silica and a solid-core-based C18 column). The methods were validated and a suitable sensitivity was found for all compounds in
the concentration range 1–100 lg L–1 (R2 > 0.999). High levels of repeatability and intermediate precision
(CV less than 0.25% and 1.7% on average for the retention time and the signal area, respectively) were obtained. The new method was applied to the determination tryptophan and its derivatives in black pig- mented glutinous and non-glutinous rice grain samples
