Karya
Judul/Title Morphometric Variation of Phytophthora palmivora Causing Black Pod Rot Disease on Cocoa (Theobroma cacao L.) in Indonesia
Penulis/Author MASANTO (1); Dr. Ir. Arif Wibowo, M.Agr.Sc. (2); Prof. Dr. Ir. Siti Subandiyah, M.Agr.Sc. (3); Koji Kageyama (4)
Tanggal/Date 2019
Kata Kunci/Keyword
Abstrak/Abstract Black pod rot disease caused by Phytophthora palmivora is one of factors contributing in decrease of cocoa production in Indonesia. This research was conducted to study the variation of P. palmivora causing black pod rot disease in Indonesia based on morphological characteristics and sexual reproduction. Materials and Methods: Pathogen was isolated from cocoa pods showing black rot symptoms in Indonesia for morphological and sexual characterization as well as molecular confirmation. Quantitative data of sporangial and sexual features were recorded and arranged in multivariateas well as calculated using phonetic method with hierarchical cluster. Those characters were clustered through average linkage and reconfirmed with principal component analysis (PCA) and dendogram using NTsys 2.10e program. Results: Fifty-five isolates of pathogenic agents had been successfully collected from 38 regencies (23 provinces) in Indonesia. They had various sporangial shape, i.e., distorted, ellipsoid, globose, obpyriform and ovoid as well as and spherical chlamydospores. These features were recognized as the characteristics of Phytophthora palmivora. Molecular analysis confirmed that all isolates were positively detected with multiplex PCR using species-specific primers. Sexual reproduction was characterized with the formation of amphyginous antheridia as well as spherical oogonia and oospores in which A2 type occurred more frequently thanA1 type. Conclusion: Those isolates were classified into 8 clusters which were independent with geographical area and mating types.
Rumpun Ilmu Ilmu Hama dan Penyakit Tanaman
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1Masanto et al 2019 Morphometric P_palmivora.pdf[PAK] Full Dokumen
2Cek Similarity_Morphometric Variation of Phytophthora palmivora Causing Black Pod Rot Disease on Cocoa (Theobroma cacao L) in Indonesia.pdf[PAK] Cek Similarity