Morphological and molecular identification of Fusarium spp. isolated from maize kernels in Java and Lombok, Indonesia
Penulis/Author
Prof. Ani Widiastuti, S.P., M.P., Ph.D. (1); Monica Lucky Karlina (2); Kurnia Ritma Dhanti (3); yufita dwi chinta (4); Prof. Dr. Tri Joko, S.P., M.Sc. (5); Dr. Suryanti, S.P., M.P. (6); Dr. Ir. Arif Wibowo, M.Agr.Sc. (7)
Tanggal/Date
2020
Kata Kunci/Keyword
Abstrak/Abstract
Fungal contamination of maize is a serious problem in Indonesia. Fusarium spp. infect maize in the field will be continuing to contaminate in the post-harvest period even though disease symptoms are not always emerged. Some Fusarium spp. produced mycotoxins which are harmful to human and animal health. Aims of this research were to reveal the presence of Fusarium spp. from both symptomatic and
unsymptomatic maize, and to identify them based on morphological characteristics and molecular analysis. Samples of maize were collected from maize cultivation areas in East Java (EJ), Central Java (CJ), West Java (WJ), Yogyakarta Special Province (DIY), and Lombok, West Nusa Tenggara. Fusarium spp. were isolated in a single spore method and cultured in potato dextrose agar (PDA) medium for morphological identification of macro-and microconidia. Molecular identification was conducted by PCR assay using
species-specific primers. Furthermore, unidentified species were analyzed by DNA sequence. This research found four species of mycotoxigenic Fusarium isolated from maize-based on molecular identification, which were Fusarium verticillioides (15 isolates), F. proliferatum (6 isolates), F. graminearum (1 isolate) and F. asiaticum (1 isolate). This research showed a novel report of F. asiaticum infection on maize kernel in Indonesia.
Rumpun Ilmu
Ilmu Hama dan Penyakit Tanaman
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
ani_biodiversitas.pdf
[PAK] Full Dokumen
2
AW20200602.pdf
3
surat pernyataan authorship_Biodiversitas.pdf
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Similarity_biodiversitas Ani.pdf
[PAK] Cek Similarity
5
2020_Biodiversitas_Fus_ani - Full Doc.pdf
[PAK] Full Dokumen
6
BuktiKorespondensi_Morphological and molecular identification of Fusarium spp_ isolated from maize kernels.pdf
[PAK] Bukti Korespondensi Penulis
7
Cek Similarity_Morphological and molecular identification of Fusarium spp_ isolated from maize kernels in Java and Lombok, Indonesia.pdf
