Karya
Judul/Title Morphological and Molecular Characterization of Rhizoctonia solani Isolates from Two Different Rice Varieties
Penulis/Author AISYAH SURYA BINTANG (1) ; Dr. Ir. Arif Wibowo, M.Agr.Sc. (2); Prof. Ir. Achmadi Priyatmojo, M.Sc., Ph.D. (3); Prof. Dr. Ir. Siti Subandiyah, M.Agr.Sc. (4)
Tanggal/Date 2017
Kata Kunci/Keyword
Abstrak/Abstract Six isolates of Rhizoctonia solani, i.e. two isolates collected from infected rice plants and four isolates from laboratory collection were studied by using morphological characters and molecular analysis. Un-weighted pair group method with arithmetic mean dendogram constructed based on cluster analysis showed that these isolates were grouped into three clusters at the 0.77 similarity coefficient. Cluster I consisted of BA, BNJ, and NBR isolates with 100% similarity and indicated that those were from AG 1 IA sub group, cluster II consisted of BND, and cluster III consisted of SL1 and SL2. Mycelium was very light brown or whitish with few and moderate sclerotia except SL1 and SL2. Molecular characterization showed that BA, BNJ, and NBR were amplified at 140 bp using Rs1F/Rs2R specific primer for R. solani AG1 IA. All isolates were amplified between 350?400 bp using Rhsp1 primer, meanwhile SL1 and SL2 were not amplified using AG2sp and AG22sp2 primers. Based on Maximum Likelihood tree analysis showed that SL1 and SL2 had high similarity based on ITS sequence data.
Rumpun Ilmu Ilmu Hama dan Penyakit Tanaman
Bahasa Asli/Original Language English
Level Nasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
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