| Penulis/Author |
TRI FITRI YANA UTAMI (1); Muhammad Novrizal Abdi Sahid, S.Farm., M.Eng., Ph.D., Apt. (2) ; Prof. Dr. Ika Puspita Sari, S.Si., M.Si., Apt. (3); Prof. Dr. Dra. apt. Ediati, SE. (4) |
| Abstrak/Abstract |
Ulcerative Colitis (UC) is a chronic inflammatory disorder affecting the colon, characterized by recurrent episodes and involving multifaceted etiological factors in its pathogenesis. This study aims to explore the pharmacological effect of Morinda citrifolia fruit ethanolic-extract (MFE) in UC and to conduct histological analyses via Hematoxylin and Eosin (HE) staining. The UC mice model was induced using 2?etic Acid administered rectally. Subsequently, mice were categorized into six groups, comprising a normal control, negative control, positive control, and three treatment groups (100mg/kgBW;200mg/kgBW;400mg/kgBW).The crude MFE exhibited notable antioxidant activity, with an IC50 value of 0.075 mg/ml. Notably, MFE administration led to a discernible reduction in Disease Activity Index (DAI) scores, with doses of 100mg/kgBW, 200mg/kgBW, and 400 mg/kgBW demonstrating better results compared to the negative control from day 6 through day 11. However, while the MFE doses showed efficacy, they did not surpass the effectiveness of the positive control group. Furthermore,immune cell infiltration within colonic tissue was markedly attenuated in the MFE-treated group compared to the negative control group. Evaluation of colon tissue damage revealed scores of 5.7, 4.2, and 3 for MFE doses of 100mg/kg, 200mg/kg, and 400mg/kg, respectively, whereas the positive control group exhibited a score of 3, indicating superior tissue preservation. Notably, these scores contrasted starkly with the negative control group, which registered a damage score of 11.8. These findings confirm our hypothesis that MFE holds promise as a therapeutic intervention for UC owing to its pharmacological properties, underscoring its potential as a natural remedy in UC management. |
