Karya
Judul/Title Molecular Identification of Bactrocera sp. Fruit Fly from Muria Forest, Central Java, Indonesia
Penulis/Author Diyah Rini Indriyanti (1) ; Dr. Suputa, S.P., M.P. (2); S.N. Jannah (3)
Tanggal/Date 4 2017
Kata Kunci/Keyword
Abstrak/Abstract Bactrocera is one of important fruit flies. There are many Bactrocera species attack fruits consumed by human or not. We found Bactrocera sp. from forest that attack the fruit that not consumed by human (wild fruit), the species is still doubtful. The morphological identification show that Bactrocera sp has most of the same morphological characters with Bactrocera calumniata and has the same wings character with the B. cucurbitae. The study aimed to confirm the taxonomic status of Bactrocera sp using identify the cytochrome oxidase I gene of mitochondrial DNA and its phylogenic. The methods included fresh larvae DNA isolation, polymerase chain reaction (PCR), electrophoresis, and sequencing. Data analysis using BLAST program and MEGA software version 6.06 programs. The results showed that sequence (435 bp) of the Bactrocera sp. had highest similarity to B. cucurbitae (100%) (GenBankAcc Number DQ006875.1), and 96% homology with B. calumniata (96%) (GenBankAcc Number GQ154088.1). The Phylogenetic clearly showed that Bactrocera sp have the same common ancestor that came from Switzerland B. cucurbitae.
Rumpun Ilmu Ilmu Hama dan Penyakit Tanaman
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1INDRIYANTI, SUPUTA, JANNAH 2017_ Molecular Identification of Bactrocera sp.pdf
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