| Abstrak/Abstract |
Sugarcane (Saccharum officinarum L.) presents high genetic complexity, posing
challenges in breeding efforts aimed at developing superior hybrids. This research utilizes DNA
barcoding, specifically leveraging the ribulose-1,5-biphosphate carboxylase-oxygenase large
subunit (rbcL) gene as a DNA barcode. This research aims to assess the effectiveness of the rbcL
gene in distinguishing local sugarcane cultivars and construct a phylogenetic tree to clarify their
evolutionary relationships. The rbcL gene was amplified in 19 samples collected from several
regions in Indonesia through PCR methods. DNA alignment, sequencing, and analyses were
conducted using Maximum Likelihood (ML) and Bayesian Inference (BI) evolutionary models.
Additionally, validation was performed using genetic distance, haplotype network, and principal
coordinate of analysis (PCoA). The results established successful amplification and identification
of samples within the Saccharum genus. Notably, samples BKS2, BKS7, OP3, NX04, Pringu, and
Kidang Kencana displayed distincy characteristic in both ML and BI analyses, substantiated by
polymorphic site variations and genetic distance assessments. However, the limitations
associated with the rbcL gene constrained comprehensive analysis of other local sugarcane
samples, hindering precise phylogenetics placement of the other samples. |
