| Abstrak/Abstract |
Chili (Capsicum annuum L.) is a self-pollinated crop, with natural cross-pollination occurring below
4%–5%. It intends to have low heterosis. Developing cross-pollination in chili currently receives much
attention to achieve diversity in trait improvement. Double-crossing becomes one of the alternatives
to achieving this goal. In this study, three different parental chili genotypes (K, B, and T) gained
crossing, with four populations (S2 K, F3 KB, F2 BTKB, and F2 KBBT) developed. Using 11 selected
sequence-related amplified polymorphism (SRAP) combination markers that target Open Reading
Frame (ORF) regions assessed molecular diversity in these chili populations. Results revealed the
possibility of identifying diversity using SRAP markers based on primer profile information. The iMEC
analysis showed high values of PIC (0.3381), discriminant power (0.882), and mean polymorphic
value (97.88%). The highest similarity emerged between the populations BTKB and KBBT as the
reciprocal. Then, the smallest similarity appeared between K and the double cross. Compared with the
self-pollinated genotype, SRAP primers discovered that double crosses provided more variation based
on Shannon's index (I) and percentage of polymorphic loci (PPL). The genetic distance denotes
maternal inheritance or extraneous involvement in progeny. However, multiple-parent hybridization
authenticated the boost in genetic diversity. |
