Transcription factor gene family of NAC (NAM, ATAF, CUC) is tightly involved in plant
development and in the response to stresses. In this study, we reported the isolation and the
characterization of NAC gene homolog from four foxtail millet genotypes. Band with approximately 1300
bp size was successfully amplified from the genomic DNA of four foxtail millet genotypes (ICERI-4,
ICERI-5, ICERI-6 and ICERI-10) using gene specific primer. The fragment was designated as SiNAC065
after showing high similarity with NAC gene homologs in the GenBank. Sequence analysis results showed
that the SiNAC065 genes isolated from the four genotypes were 1265 bp in length with one intron and
two exons. The two exons encode 325 amino acids with the conserved domain located between amino
acid 19-325. The SiNAC065 protein identified in this study have 8 conserved motives in the conserved
region which categorized them as SNAC (stress responsive NACs) orthologs that are involved in the
abiotic stress responses. Different features of SiNAC065 isolated from the tolerant- and the sensitivegenotypes should provide information of the gene’s role in salinity tolerance mechanism of foxtail millet.
Rumpun Ilmu
Pemuliaan Tanaman
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Molecular cloning and characterization of NAC genes from four foxtail millet genotypes.pdf
