| Abstrak/Abstract |
Aims: To identify mold contaminant on salted fish, from two different market locations (Kenjeran market, Surabaya and
Beringharjo market, Yogyakarta). Furthermore, levels of AFB1 (aflatoxin B1) in salted fish samples were assayed.
Methodology and results: The samples were cultivated on DRBC (Dichloran Rose Bengal Chloramphenicol Agar) and
DG-18 (Dichloran (18%) Glycerol Agar) medium for enumeration, then transferred on MEA (Malt Extract Agar) medium
for isolation and identification, followed by ELISA test to measure the AFB1 level. Meanwhile aflatoxin biosynthesis
correlated genes (i.e. aflR, nor-1 and omtB genes) were identified using Polymerase Chain Reaction (PCR) method. The
results showed that Aspergillus tamarii and A. flavus being contaminant on salted fish along with A. sydowii, A. niger, A.
versicolor, Penicillium citrinum, and P. chrysogenum. Rhizopus sp. contamination was also found. AFB 1 was positively
detected in all of samples with the highest concentration measured was 75.81 µg/kg which belong to Lidah salted fish
and the lowest concentration measured was 4.33 µg/kg which belong to Rese salted fish. The suspected A. flavus and
A. tamarii isolated from salted fish was positively detected in the presence of aflR, nor-1 and omtB genes.
Conclusion, significance and impact of study: Mold contamination was detected in salted fish from two different
markets and all of those samples were contaminated by AFB1. These can be important information related to food
safety aspect for salted fish.
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