Karya
Judul/Title MiR-141-3p Relative Expression Level from FFPE Samples as Biomarker of Prostate Adenocarcinoma Carcinogenesis in Yogyakarta, Indonesia
Penulis/Author Sari Eka Pratiwi (1) ; Sri Nuryani Wahyuningrum (2); Rachma Greta Perdana Putri (3); Dr. dr. R. Danarto, Sp.B., Sp.U(K). (4); dr. Didik Setyo Heriyanto, Sp.PA(K)., Ph.D (5); dr. Nur Arfian, Ph.D. (6); Prof. dr. Sofia Mubarika Harjana, M.Med.Sc., Ph.D. (7); dr. Indwiani Astuti Dr.Med. (8)
Tanggal/Date 28 2022
Kata Kunci/Keyword
Abstrak/Abstract Globally, prostate cancer (PCA) is the second leading cause of male cancer-associated mortality. Micro-RNAs (miRNAs) are small non-coding RNAs considered promising biomarkers for diagnosis, prognosis, and treatment options. A miR-141 expression is frequently dysregulated and influences the development and progression of PCA. This study aimed to identify miR-141 expression level as a marker to differentiate PCA from another prostate anomaly, especially in Yogyakarta- Indonesia. Formalin-fixed paraffin-embedded (FFPE) tissues for each three groups: benign prostatic hyperplasia/BPH, high-grade prostatic intraepithelial neoplasia/HGPIN, and PCA (n=7/group) were stored in a commercial clinical laboratory in Yogyakarta. The total RNA was extracted from FFPE sections using miRNeasy FFPE kit, followed by the quantification of miR-141-3p expression level by RT-PCR. The result showed that miR- 141 relative expression level on PCA was higher than other groups and significantly different (P<0.05, Kruskal Wallis test). The mean of the miR-141 relative expression level of BPH, HGPIN, and PCA were 1.04±0.87, 6.44±7.8, and 7.06±8.83, respectively. The relative expression level of miR-141 can potentially be a prognostic biomarker in PCA and could differentiate aggressiveness in prostate anomaly, especially BPH, HGPIN, and PCA.
Rumpun Ilmu Bedah (Umum, Plastik, Orthopaedi, Urologi, Dll)
Bahasa Asli/Original Language English
Level Nasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
12355-Article Text-13795-2-10-20220427.pdfBukti Published
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