| Abstrak/Abstract |
Phalaenopsis“Sogo Vivien” is an orchid hybrid with mini size plant
body, and exhibits numerous beautiful pink flowers, that is ideal as ornamental
pot plant. Some plants of this orchid exhibit variegated leaves that improve
the beauty of the plant, not only because of the flower but also as attracted
leaves. This orchid has high economical value, but mass propagation of this
orchid has not established yet. An effective method to propagate both the
normal and variegated plants is worth to be generated. The objective of this
research was to produce a large number of P. “Sogo Vivien” plants, including
the variegated plants. The method used seeds from self pollinating variegated
plant, and flower stalk nodes. The seeds were sown on three various medium:
VW, NP and MS, and flower stalk nodes were planted on VW + BA 10 mg l-1 +
active carbon. The results showed that the best medium for in vitro culture of
P. “Sogo Vivien” was NP medium, in which all seeds could grew into plantlets.
Most plantlets emerged from the seeds were non variegated, only one plantlet
out of 1344 seeds was variegated (0.007%). Although all emerged plantlets
from flower stalk exhibited variegated leaves. Particularly, the plantlets arised
from the second and third basal nodes of flower stalk showed the highest
growth rate than that from the other nodes. Histological analysis showed that
at 11-13 days after shoot segment plantation on NP medium, the shape of
apical cells in the nodes was changed, then followed by the change of cell
shape in the basal part of the nodes, produced bipolar pattern, then gradually
developed into shoot. These results suggest that mass propagation could be
achieved using seed culture, but to get the variegated phenotypes, the second
and third nodes of flower stalk from variegated plant were the best explants
to be used |
